| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Effect of an oversulfated exopolysaccharide on angiogenesis induced by fibroblast growth factor-2 or vascular endothelial growth factor in vitro
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| Autores: |
Matou, Sabine
Colliec-jouault, Sylvia
Galy Fauroux, Isabelle
Ratiskol, Jacqueline
Sinquin, Corinne
Guezennec, Jean
Fischer, Anne-marie
Helley, Dominique
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| Data: |
2005-03
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| Ano: |
2005
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| Palavras-chave: |
Matrigel
Collagen gel
Alpha 6 integrin subunit
Angiogenic growth factor
Polysaccharide
Angiogenesis
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| Resumo: |
The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6) integrin subunit expression, which was enhanced by 50% in the presence of the growth factors. Indeed, a monoclonal anti-alpha(6) blocking antibody abolished this vascular tube formation. EPS had no effect in any of the experimental conditions, underlying the importance of sulfation in the angiogenic effects of exopolysaccharide. By potentiating the angiogenic activity of FGF-2 and/or VEGF, OS-EPS, which possesses low anticoagulant activity and thus a low hemorrhagic risk, could potentially be used to accelerate vascular wound healing or to promote the growth of collateral blood vessels in ischemic tissues. (C) 2004 Elsevier Inc. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2005/publication-2118.pdf
DOI:10.1016/j.bcp.2004.11.021
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2118/
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| Formato: |
application/pdf
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| Fonte: |
Biochemical Pharmacology (0006-2952) (Elsevier), 2005-03 , Vol. 69 , N. 5 , P. 751-759
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| Direitos: |
2004 Elsevier Inc. All rights reserved.
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